Exposure to Electromagnetic Field during Gestation Adversely Affects the Electrophysiological Properties of Purkinje Cells in Rat Offspring

M Haghani, V Pouladvand, S. M. J Mortazavi, M Razavinasab, M Bayat, M Shabani

Abstract


Background: Prenatal adverse effects of radiofrequency electromagnetic fields (RF-EMF)  exposure on nervous system are a issue of major concern. Thus, in this study we evaluated the membrane current flow properties of Purkinje neurons after maternal exposure to  900 MHz pulsed RF-EMF.

Methods: During all days of pregnancy, rats in the EMF-exposed group were exposed to 900 MHz pulsed-EMF radiation for 6 h per day. The effects of RF-EMF exposure on the electrophysiological properties of the Purkinje cerebellum neurons from male pups were evaluated by whole-cell patch clamp recordings in current and voltage clamp modes. In voltage-clamp experiments, the holding potential was -60mV, and a depolarizing voltage step (1000ms duration) was applied from -60  to +50 mV in 10 mV increments at 2s intervals.

Results: The exposure group demonstrated reduced spontaneous firing associated with upward and rightward shift in I/V curve compared to the control rats. Moreover, the peak amplitude of the current for the exposure pups also revealed a significant decrement. The reversal potential was +40 mV and +20 mV for the control and RF-EMF groups, respectively and showed significant differences between the two groups.

Conclusion: The decrease in ion's conductance could be attributed to the observed decrease in the voltage onset of the inward current, peak amplitude and voltage shift. 


Keywords


Key words: Mobile phone, EMF, Purkinje cells, Electrophysiologicla properties, Prenatal

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DOI: https://doi.org/10.22086/jbpe.v0i0.560

eISSN: 2251-7200        JBPE NLM ID: 101589641

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